Endonukleaza specifična za strukturu prepusta 1
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| Identifikatori | |||||||||||||||||||||||||
| Alijasi | FEN1 | ||||||||||||||||||||||||
| Spoljašnji ID | OMIM: 600393 MGI: 102779 HomoloGene: 3034 GeneCards: FEN1 | ||||||||||||||||||||||||
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| Vrste | Čovek | Miš | |||||||||||||||||||||||
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| Location (UCSC) | n/a | Chr 19: 10.18 – 10.18 Mb | |||||||||||||||||||||||
| PubMed search | [2] | [3] | |||||||||||||||||||||||
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Flap endonukleaza 1 je enzim koji je kod ljudi kodiran FEN1 genom.[4][5]
Protein kodiran ovim genom uklanja 5' prepuste tokom DNK popravke i obrađuje 5' krajeve Okazakijevih fragmenta pri sintezi zaostajućeg lanca DNK. Direktna fizička interakcija između tog proteina i AP endonukleaze 1 tokom popravke isecanjem dugog segmenta baza omogućava koordinirani pristup proteina supstratu, čime se supstrat dodaje od jednog enzima do drugog. Ovaj protein je član XPG/RAD2 familije endonukleaza i jedan je od deset proteina koji su esencijalni za bezćelijsku replikaciju DNK. Sekundarna struktura DNK može da inhibira flap obradu na pojedinim trinukleotidnim ponavljanjima u maniru zavisnom od dužine koji skrivaju 5' kraj prepusta, koji je neophodan za vezivanje i presecanje proteinom kodiranim ovim genom. Stoga sekundarna struktura može da onemogući protektivnu funkciju ovog proteina, što dovodi do trinukleotidnih ekspanzija specifičnih za mesto.[5]
Interakcije
Endonukleaza 1 specifična za flap strukturu formia interakcije:
Reference
- ^ а б в GRCm38: Ensembl release 89: ENSMUSG00000024742 - Ensembl, May 2017
- ^ „Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ „Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Hiraoka LR, Harrington JJ, Gerhard DS, Lieber MR, Hsieh CL (1995). „Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human”. Genomics. 25 (1): 220—5. PMID 7774922. doi:10.1016/0888-7543(95)80129-A.
- ^ а б „Entrez Gene: FEN1 flap structure-specific endonuclease 1”.
- ^ а б Dianova II, Bohr VA, Dianov GL (2001). „Interaction of human AP endonuclease 1 with flap endonuclease 1 and proliferating cell nuclear antigen involved in long-patch base excision repair”. Biochemistry. 40 (42): 12639—44. PMID 11601988. doi:10.1021/bi011117i.
- ^ а б Sharma S, Sommers JA, Wu L, Bohr VA, Hickson ID, Brosh RM (2004). „Stimulation of flap endonuclease-1 by the Bloom's syndrome protein”. J. Biol. Chem. 279 (11): 9847—56. PMID 14688284. doi:10.1074/jbc.M309898200.
- ^ а б в Henneke G, Koundrioukoff S, Hübscher U (2003). „Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation”. Oncogene. 22 (28): 4301—13. PMID 12853968. doi:10.1038/sj.onc.1206606.
- ^ а б Hasan S, Stucki M, Hassa PO, Imhof R, Gehrig P, Hunziker P, Hübscher U, Hottiger MO (2001). „Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300”. Mol. Cell. 7 (6): 1221—31. PMID 11430825. doi:10.1016/s1097-2765(01)00272-6.
- ^ Chai Q, Zheng L, Zhou M, Turchi JJ, Shen B (2003). „Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment processing during Okazaki fragment maturation”. Biochemistry. 42 (51): 15045—52. PMID 14690413. doi:10.1021/bi035364t.
- ^ Jónsson ZO, Hindges R, Hübscher U (1998). „Regulation of DNA replication and repair proteins through interaction with the front side of proliferating cell nuclear antigen”. EMBO J. 17 (8): 2412—25. PMC 1170584
. PMID 9545252. doi:10.1093/emboj/17.8.2412. - ^ Gary R, Ludwig DL, Cornelius HL, MacInnes MA, Park MS (1997). „The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21”. J. Biol. Chem. 272 (39): 24522—9. PMID 9305916. doi:10.1074/jbc.272.39.24522.
- ^ Chen U, Chen S, Saha P, Dutta A (1996). „p21Cip1/Waf1 disrupts the recruitment of human Fen1 by proliferating-cell nuclear antigen into the DNA replication complex”. Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11597—602. PMC 38103 . PMID 8876181. doi:10.1073/pnas.93.21.11597.
- ^ Yu P, Huang B, Shen M, Lau C, Chan E, Michel J, Xiong Y, Payan DG, Luo Y (2001). „p15(PAF), a novel PCNA associated factor with increased expression in tumor tissues”. Oncogene. 20 (4): 484—9. PMID 11313979. doi:10.1038/sj.onc.1204113.
- ^ Brosh RM, von Kobbe C, Sommers JA, Karmakar P, Opresko PL, Piotrowski J, Dianova I, Dianov GL, Bohr VA (2001). „Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity”. EMBO J. 20 (20): 5791—801. PMC 125684 . PMID 11598021. doi:10.1093/emboj/20.20.5791.
Literatura
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